Journal: Leukemia

Article Title: Regulation of PI3K signaling in T cell acute lymphoblastic leukemia: a novel PTEN/Ikaros/miR-26b mechanism reveals a critical targetable role for PIK3CD

doi: 10.1038/leu.2017.80

Figure Lengend Snippet: A: miRNA profile of Array data. KO576, KO577, KO578 and KO579 are Pten -knockout mouse T-ALL samples. WT580 and WT581 are wild-type mouse thymocytes. B: Decreased miR-26b expression level in mouse Pten deficient T-ALL cell lines (LPN248, LPN236 and LPN228) compared with mouse Ink4a/Arf knock-out T-ALL cell line (LPN211) and mouse wild type thymocytes (P<0.001). C. Decreased miR-26b in human T-ALL cell lines, CCRF-CEM, SUPT1, LOUCY, KOPT-K1, JURKAT and MOLT4 compared with postnatal normal human thymus (P<0.001). D: Decreased expression level of miR-26b in human primary T-ALL samples (P<0.001). E. Correlation of miR-26b expression level with PTEN level in human primary T-ALL samples (r=0.3987, P=0.039).

Article Snippet: Hybridization of biotin-labeled cDNA was carried out on a miRNA microarray chip (MD Anderson miRNA expression Bioarray Version 5), which contains 2300 miRNA probes, including 1400 human and 900 mouse miRNA genes, in duplicate.

Techniques: Knock-Out, Expressing

Journal: PLoS ONE

Article Title: Complement System in the Pathogenesis of Benign Lymphoepithelial Lesions of the Lacrimal Gland

doi: 10.1371/journal.pone.0148290

Figure Lengend Snippet: The results of differences in gene expression from microarray and RT-PCR were found to be consistent.

Article Snippet: Human genome microarray (version 5.0; Phalanx Biotech Group); RNA amplification kit (New England Biolabs, Ipswich, MA, USA); Agilent gene hybridization buffer kit (New England Biolabs, Ipswich, MA, USA); hybridization kit (New England Biolabs, Ipswich, MA, USA); RNA extraction and reverse transcription kit (New England Biolabs, Ipswich, MA, USA) were used in this study.

Techniques: Gene Expression, Microarray, Reverse Transcription Polymerase Chain Reaction

Journal: EMBO Reports

Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA

doi: 10.1038/s44319-024-00339-8

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Images were acquired and quantified using the ChemiDoc TouchImaging System (Bio-Rad) and the software ImageLab versions 5.2.1 or 6.1.0 (Bio-Rad).

Techniques: CRISPR, Bacteria, Recombinant, Cloning, Clone Assay, Mutagenesis, Control, Plasmid Preparation, Sequencing, Luciferase, Gene Expression, Labeling, Binding Assay, Modification, Saline, Western Blot, Transfection, Protease Inhibitor, Random Hexamer, Reverse Transcription, Membrane, In Situ, Proximity Ligation Assay, SYBR Green Assay, Microarray, Software